Synergistic Effects of Electrical Stimulation and Aligned Nanofibrous Microenvironment on Growth Behavior of Mesenchymal Stem Cells.

Abstract

Incontrollable cellular growth behavior is a significant issue, which severely affects the functional tissue formation and cellular protein expression. Development of natural extracellular matrix (ECM) like biomaterials to present microenvironment cues for regulation of cell responses can effectively overcome this problem. The external simulation and topological characteristics as typical guiding cues are capable of providing diverse influences on cellular growth. Herein, we fabricated two-dimensional aligned conductive nanofibers (2D-ACNFs) by an electrospinning process and surface polymerization, and the obtained 2D-ACNFs provided the effects of both alignment and electrical stimulation (ES) on cellular response of human mesenchymal cells (hMSCs). The results of cellular responses implied that the obtained 2D-ACNFs could offer a synergistic effect of both ES and aligned nanopattern on hMSC growth behavior. The effects could not only promote hMSCs to contact each other and maintain cellular activity but also provide positive promotion to regulate cellular proliferation. Thus, we believe that the obtained 2D-ACNFs will have a broad application in the biomedical field, such as cell culture with ES, directional induction for cell growth, and damaged tissue repair, etc.