Synergistic Effect ofTaxolandVincristineAgainstMCF-7 Cell Line

Abstract

Background: Women worldwide are concerned about breast cancer, resulting in high mortality and morbidity. Objectives: In this research, to investigate the function of Taxol, Vinblastine, and Vincristine on the MCF-7 cell line, different concentration levels (10, 20, 40, 80, 160, and 320 µmol/mL) of the drugs were examined. Taxolwas also used in combination with Vinblastine and Vincristine. Methods: The examined compounds were applied to the cells for 48 h. The MTT test was employed for assessing cytotoxicity, and the nonlinear regression method was run to estimate the values of IC50 (half maximal inhibitory concentration) values. Results: It was found that taxol + vincristine, taxol, and vinblastine treatments with concentrations of 41.45, 64.46, and 67.12 µmol/mL resulted in the best 50% inhibitory effect. The lowest effective concentration that had significant effects on cancer cells was vinblastine (160 µmol/mL), taxol + vincristine (80 µmol/mL), taxol (80 µmol/mL), taxol + vinblastine (80 µmol/mL), and vincristine (40 µmol/mL), respectively (P < 0.05). Conclusions: From the results of the current study, a synergistic reaction was found between taxol and vincristine based on calculated CI values.