Abstract
Nine day-old seedlings of Pisum sativum (var. Feltham First) were fumigated with sulphur dioxide, nitrogen dioxide, or a combination of both, for six days. A comparison of the activities of ribulose diphosphate carboxylase (RuDPC), glutamate-pyruvate transaminase (GPT), glutamate-oxaloacetate transaminase (GOT) and peroxidase enzymes in extracts from fumigated seedlings showed various differences with respect to those of control seedlings. In some cases the threshold concentration of a pollutant required to produce a change in enzyme activity was lowered when combinations of pollutants were used. A synergistic effect between the pollutants was clearly reflected in the changes in enzyme activity of RuDPC ( P < 0·01) and peroxidase ( P < 0·001) at concentrations within the ranges 0–2 ppm SO 2 and 0–0·1 ppm NO 2 and also peroxidase ( P < 0·001) at concentrations within the ranges 0–0·2 ppm SO 2 and 0–1 ppm NO 2.
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