Synergistic Effect of Rubus crataegifolius and Ulmus macrocarpa Against Helicobacter pylori Clinical Isolates and Gastritis.

Jung Uoon Park,Young Hee Jo,Hyun Kyu Kim,Young Ik Lee,Md Aziz Abdur Rahman,Jin Sook Cho,Jong Seok Kim

doi:10.3389/fphar.2020.00004

Abstract

Helicobacter pylori is one of the most widespread infections involved in the pathogenesis of chronic gastritis, peptic ulcer, and gastric cancer. Hence, there is an urgent need to develop medications against H. pylori. This study aimed to evaluate synergistic effect of Rubus crataegifolius (RF) and Ulmus macrocarpa Hance (UL) against H. pylori. Antibacterial susceptibility of each extract either separately or in combination was studied against two H. pylori standard strains and 11 clinical isolates using agar dilution method. The effect of the extracts on H. pylori inoculated Balb/c mice model was also studied using single dosing (100 mg/kg each) approach. The MIC50 of RF and UL were more than 100 and 200 µg/ml, respectively, against the tested strains. However, simultaneous treatment with RF and UL at 75 and 50 µg/ml, respectively, showed decreased viable cell number, MIC70, and at 75 µg/ml each showed synergic effect with MIC90. On H. pylori inoculated Balb/c mice model, RF and UL separately (100 mg/kg each) showed moderate anti-H. pylori effect, while simultaneous treatment of RF and UL with same dose showed significant synergistic anti-gastric effects in stomach. The results showed a significant synergistic effect of plants extract against H. pylori infection and eventually gastric mucosal damage. Our finding could be considered a valuable support in the treatment of H. pylori induced gastritis and may contribute to the development of new and safe combined herbal product as anti-H. pylori regimens.

Highlights

Helicobacter pylori is a pathogenic bacterium that can persist in the stomach of an infected person for their entire life
We report in vitro anti-H. pylori activity of two popular Korean medicinal plants Rubus crataegifolius Bunge (RF, Family: Rosaceae) and Ulmus macrocarpa Hance (UL, Family:Ulmaceae) either separately or in combination and in vivo anti-gastritic effect of the extracts against H. pylori infected animal model
As described in Materials and Methods, the RF and UL used in the experiment were standardized by determining the major components using high performance liquid chromatography (HPLC) chromatography

Summary

Introduction

Helicobacter pylori is a pathogenic bacterium that can persist in the stomach of an infected person for their entire life. The eradication rate of standard first line therapy has fallen below 80% in many Asian countries, including China, India, and Korea (Liou et al, 2015; Liou et al, 2016b). It is noteworthy that metronidazole resistant rate was higher than 60% in Asian counties including China and India (Liou et al, 2015). In the face of the declining eradication rate of standard triple therapy, several alternative strategies have been proposed to increase the first line treatment (Rimbara et al, 2011; Gisbert and Calvet, 2012; Venerito et al, 2013). Despite advances in antimicrobial therapy, there is still no ideal treatment and indications due to increasing resistance, side effects, and falling eradication rates. Some of them including herbal treatment, novel antibiotics, or classical ones from natural product in different combinations, using probiotics, etc. (Brown and Jiang, 2013; Takeuchi et al, 2014; Goderska et al, 2018)

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