Abstract
1. 1. Bacterial lipopolysaccharide (LPS) stimulated [ 3H]TdR incorporation of rat splenocytes in a concentration dependent manner. 2. 2. Phorbol 12-myristate 13-acetate (PMA) alone has little effect on rat splenocyte proliferation but it exerted a marked synergistic effect on LPS-induced [ 3H]TdR incorporation when added at the first few hours of incubation with LPS. Minimal synergistic effect of PMA was observed if it was added later than 4 hr after LPS application. 3. 3. Both LPS-stimulated and PMA synergized incorporation of [ 3H]TdR in rat splenocytes were inhibited by H-7, a protein kinase C inhibitor. 4. 4. The results support the notion that the activation of protein kinase C is a necessary but insufficient cellular signal in the initiation of proliferative response of rat splenocytes by LPS.
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