Synergistic effect of ozone and non-oxidizing bacteriostatic agent on biofouling control of reverse osmosis membranes

Abstract

Biofouling was the critical flaw of the reverse osmosis (RO) process. Both strong oxidizing and non-oxidizing bacteriostatic agents had obvious defects facing this problem. This study firstly compared the single and synergistic effect of a strong oxidizing agent (ozone) and a commercial non-oxidizing bacteriostatic agent (FR110) on RO membrane fouling control of reclaimed water, to find a more high-efficiency and practical approach. Consequently, the synergistic application of ozone and FR110 at low dosages effectively reduced the fouling of the RO membranes (18% normalized flux alleviation than the control group). Further analysis suggested that FR110 could induce a thinner fouling layer, while ozone pretreatment could lead to a loose fouling layer. The bacterial community analyses suggested that the relative abundance of biofouling-related genera was the key reason causing fouling differences. Phenotypic prediction confirmed the advantages of the synergetic application of FR110 and O3 in reducing the biofilm-forming capacity. Pure-strain experiments showed the synergetic application of FR110 and O3 could inhibit the overgrowth and secretion of certain biofilm-forming strains, such as Pseudomonas aeruginosa PAO1. In summary, synergistic disinfection was a promising way to regulate the bacterial community structure and biofilm-forming potentials, hoping for high-efficiency biofouling control in the RO system.