Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds.

Jana Dorazilová,Silvia Kočiová,Radek Veselý,Vojtěch Adam,Kristýna Šmerková,Lucy Vojtová,Johana Muchová,Pavel Diviš,Veronika Pavliňáková,Pavel Kopel

doi:10.3390/nano10101971

Abstract

A highly porous scaffold is a desirable outcome in the field of tissue engineering. The porous structure mediates water-retaining properties that ensure good nutrient transportation as well as creates a suitable environment for cells. In this study, porous antibacterial collagenous scaffolds containing chitosan and selenium nanoparticles (SeNPs) as antibacterial agents were studied. The addition of antibacterial agents increased the application potential of the material for infected and chronic wounds. The morphology, swelling, biodegradation, and antibacterial activity of collagen-based scaffolds were characterized systematically to investigate the overall impact of the antibacterial additives. The additives visibly influenced the morphology, water-retaining properties as well as the stability of the materials in the presence of collagenase enzymes. Even at concentrations as low as 5 ppm of SeNPs, modified polymeric scaffolds showed considerable inhibition activity towards Gram-positive bacterial strains such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis in a dose-dependent manner.

Highlights

The skin is the largest organ of the human body, covering on average an area of 1.8 m2 with the main functions of protection, respiration, and homeostasis maintenance [1]
Overuse of antibiotics has led to the formation of a strain resistant to β-lactam-derived antibiotics, so-called methicillin-resistant Staphylococcus aureus (MRSA) [8]
We aimed to present a comprehensive study of the impact of antibacterial additives on collagen-based materials and promote the application of nanoparticles in tissue engineering

Summary

Introduction

The skin is the largest organ of the human body, covering on average an area of 1.8 m2 with the main functions of protection, respiration, and homeostasis maintenance [1]. Minor skin wounds follow the healing process of homeostasis, inflammation, proliferation, and matrix deposition with the remodeling of the tissue as the final result [2]. Severely infected wounds larger than 2 cm in diameter follow a slower and more complicated repair process and often fail closure [3,4,5]. Opportunistic skin bacteria from the genus Staphylococcus are a common source of infection, namely, Staphylococcus aureus or Staphylococcus epidermidis [6,7]. Proper antibacterial wound dressing can support healing of chronic wounds. Tissue engineering (TE) could step-up production of an antibacterial material that could provide the necessary healing support and antimicrobial environment for complex wound management [9,10,11,12]

Objectives

Methods

Results