Synergistic effect of biological and advanced oxidation process treatment in the biodegradation of Remazol yellow RR dye

Muruganandham Thanavel,Sanjay Prabhu Govindwar,Ramu Selvam,Paul Olusegun Bankole,Senthil Kumar Sadasivam

doi:10.1038/s41598-020-77376-5

Abstract

The current study investigated the efficiency of synergistic biological and Advanced Oxidation Process (AOPs) treatment (B-AOPs) using Aeromonas hydrophila SK16 and AOPs-H2O2 in the removal of Remazol Yellow RR dye. Singly, A. hydrophila and AOPs showed 90 and 63.07% decolourization of Remazol Yellow RR dye (100 mg L−1) at pH 6 and ambient temperature within 9 h respectively. However, the synergistic B-AOPs treatments showed maximum decolorization of Remazol Yellow RR dye within 4 h. Furthermore, the synergistic treatment significantly reduced BOD and COD of the textile wastewater by 84.88 and 82.76% respectively. Increased levels in laccase, tyrosinase, veratryl alcohol oxidase, lignin peroxidase and azo reductase activities further affirmed the role played by enzymes during degradation of the dye. UV–Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), high-performance liquid chromatography (HPLC) and gas chromatography–mass spectroscopy (GC–MS) confirmed the biotransformation of dye. A metabolic pathway was proposed based on enzyme activities and metabolites obtained after GC–MS analysis. Therefore, this study affirmed the efficiency of combined biological and AOPs in the treatment of dyes and textile wastewaters in comparison with other methods.

Highlights

The current study investigated the efficiency of synergistic biological and Advanced Oxidation Process (AOPs) treatment (B-advanced oxidation process (AOP)) using Aeromonas hydrophila SK16 and AOPs-H2O2 in the removal of Remazol Yellow RR dye
The biologically treated sample was centrifuged at 10,000 rpm for 15 min, and subjected to Advanced Oxidation Process (AOPs) with 4% H 2O2 for 3–6 h
The significance values obtained for the treatment with bacteria, AOPs and B-AOPs were 0.823, 0.679 and 0.903 which further affirmed the normality of the experimental data on Remazol Yellow RR dye when subjected to Shapiro–Wilk tests

Summary

Introduction

The current study investigated the efficiency of synergistic biological and Advanced Oxidation Process (AOPs) treatment (B-AOPs) using Aeromonas hydrophila SK16 and AOPs-H2O2 in the removal of Remazol Yellow RR dye. To overcome the limitation, Advanced Oxidation Process (AOPs) is usually combined with biological treatment in the presence of solar radiation for the biodetoxification of textile azo dyes. This method involves the breakdown of the dye constituents followed by removal of toxic aromatic a mines[13]. Different bacteria strains have been deployed in the combined biological and advanced oxidation process (AOPs) treatment of textile dye This is the first report on the degradation of Remazol Yellow RR dye by A. hydrophila in combination with AOPs using lesser percentage of hydrogen peroxide

Methods

Results

Discussion

Conclusion