Synergistic effect between sulfide mineral and acidophilic bacteria significantly promoted Cr(VI) reduction

Abstract

Natural pyrite was an economical choice for efficient Cr(VI) remediation, while its deep utilization was inhibited due to the passivation effect. In this study, pyrite passivation/dissolution and active sites regeneration mechanism under the activity of acidophilic bacteria with different energy metabolism characteristic in Cr(VI) reduction have been investigated. The reduction capacity was in the order of Acidithiobacillus thiooxidans, Acidithiobacillus ferrooxidans(S), Acidithiobacillus ferrooxidans(Fe), Leptospirillum ferrooxidans and chemical control. The maximal reduction efficiency was achieved in A. thiooxidans system, which is 4.5 times higher than the L. ferrooxidans system. In chemical system, sulfur and Fe(III)/Cr(III)–oxyhydroxysulphate accumulation would result in pyrite passivation. A. thiooxidans attached on pyrite surface and exerted synergistic effect on pyrite corrosion coupled with Cr(VI). Sulfur oxidation promoted proton regeneration, pyrite lattice Fe(II) dissolution and active sites regeneration, which were beneficial to sustainable Cr(VI) reduction. Secondary iron mineral formation on pyrite was accelerated with the iron oxidation bacteria activity increasing. Excessive oxidation to surface sites Fe(II) and the accumulation of S0/Sn2− led to the passivation effect in L. ferrooxidans system. Cr(VI) acquired electron from Fe(II) and disulfide and resulted in the bond break between them. The combined effect of specific sulfur oxidizing bacteria activity and Cr(VI) oxidation efficient promoted pyrite dissolution and active sites regeneration. The interaction between acidophilic bacteria and pyrite significantly enhanced Cr(VI) reduction efficiency.