Abstract
ABSTRACT Chemical and bioactive analyses of spice oils have been widely performed. However, spice blends such as curry powder are not prescribed as herbal medicine, so their bioactivities have not been sufficiently explored. Here, cinnamon, fennel, and clove were blended following the CH1 herbal medicine prescription, and, in mice, appetite-enhancing effects were compared between the blended and individual spices. GC and GC/MS analyses revealed the following major components of the CH1 blended spice oil: trans-anethole, trans-cinnamaldehyde, and eugenol. Food intake was observed in 4 h feeding tests after 16 h of fasting. Eugenol and trans-anethole lowered the dose for the appetite-enhancing effects of trans-cinnamaldehyde 10-fold. After 1 week of daily administration, the dose-lowering effect of eugenol disappeared and the appetite-enhancing effects of trans-cinnamaldehyde occurred at a higher dose. Although the dose-lowering effect of trans-anethole decreased, food intake was still increased significantly by daily administration of blended spices at the lower dose.
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