Synergistic anticancer effects of electrospun nanofiber-mediated codelivery of Curcumin and Chrysin: Possible application in prevention of breast cancer local recurrence

Abstract

To decrease the locoregional recurrence of breast cancer, the in vitro anticancer efficacy of electrospun nanofibers (NFs) co-loaded with Curcumin (Cur) and Chrysin (Chr), as natural anticancer compounds, against T47D breast cancer cells was evaluated. According to FE-SEM results, the randomly oriented bead-free CurChr co-loaded PLGA/PEG fibers presented more interconnection among the fibers with increased average diameter to around 800 nm. Also, co-loading Cur and Chr into the NFs improved mechanical properties than single drug-loaded NFs. The in vitro release profile indicated the almost identical and prolonged release profile of drugs from CurChr-NFs that were lower than that from Cur-NFs and Chr-NFs. Based on MTT, CurChr-loaded PLGA/PEG NFs displayed effectively synergistic antiproliferative activities than the single drug-loaded NFs. Among loading different non-constant ratios of Cur and Chr in polymeric NFs, 10:5% (wt:wt%) CurChr-NFs exhibited the best synergistic cytotoxicity against the cells and could further altered the mRNA levels of bax, bcl-2, p53, Cyclin D1, caspase-3, caspase-7, and hTERT genes. In conclusion, the dual drug-loaded NFs showed an excellent capacity to inhibit T47D breast cancer cells in vitro than the single drug-encapsulated NFs. Therefore, the fabricated dual drug-encapsulated NFs may achieve a safe and suitable application for breast cancer relapse rate after surgery.