Abstract
Bacteria can form biofilms, complex microbial communities protected from environmental stress, on food contact surfaces. Brassicaceae plant has been shown to contain bioactive compounds with antimicrobial activities. The objective of this study was to evaluate the synergistic effects of Brassicaceae species and proteinase K against E. coli O157:H7 biofilm. We determined the minimum biofilm inhibitory concentration, the fractional inhibitory concentration indexes, and the synergistic inhibitory effect of Raphanus sativus var. longipinnatus, R. sativus, and Brassica oleracea var. acephala extracts with proteinase K on E. coli O157:H7. The biofilm showed a 49% reduction with 2 mg/mL R. sativus. The combination of proteinase K 25 µg/mL significantly increased the effect of 2 mg/mL R. sativus var. longipinnatus and the combined treatment yielded up to 2.68 log reduction on stainless steel coupons. The results showed that the combination of R. sativus var. longipinnatus extract and proteinase K could serve as an anti-biofilm agent with synergistic effects for inhibiting E. coli O157:H7 biofilm on stainless steel surfaces.
Highlights
Escherichia coli O157:H7 is a major foodborne pathogen, which causes abdominal pain, diarrhea and even hemolytic uremic syndrome in humans w orldwide[1]
A previous study has shown that Brassicaceae plant extracts reduced E. coli O157:H7 during every step of biofilm formation[21]
Enterocin AS-48 treatment in combination with other agents such as EDTA, sodium tripolyphosphate, pH, and heat treatment in apple juice increased the permeability of planktonic E. coli O157:H7 cell m embranes[23]
Summary
Escherichia coli O157:H7 is a major foodborne pathogen, which causes abdominal pain, diarrhea and even hemolytic uremic syndrome in humans w orldwide[1]. Many studies have tested the use of disinfectants, essential oils, plant extracts, and other chemicals to prevent biofilm formation or to remove existing b iofilms[7]. Plant-derived natural agents have been studied for their potential for biofilm reduction without any resistance or residue of toxic compounds. A previous study has shown that Brassicaceae plant extracts reduced E. coli O157:H7 during every step of biofilm formation[21]. The use of antibiotics in combination with proteinase K showed stronger inhibitory effect on Staphylococcus aureus biofilm than a fivefold amount of gentamycin, streptomycin or ampicillin a lone[24]. The purpose of this study was to evaluate the combinatorial effect of Brassicaceae plant extracts such as R. sativus var. Acephala extracts with proteinase K on E. coli O157:H7 biofilm, and to evaluate their potential application to stainless steel surfaces The purpose of this study was to evaluate the combinatorial effect of Brassicaceae plant extracts such as R. sativus var. longipinnatus, R. sativus, and Brassica oleracea var. acephala extracts with proteinase K on E. coli O157:H7 biofilm, and to evaluate their potential application to stainless steel surfaces
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