Abstract
The research is aimed to assess the joint antibacterial effects of hydrogen peroxide and methanolic stem extract (SNP-MSE)-prepared silver nanoparticles against Klebsiella pneumoniae. Utilizing MSE under sunlight, biogenic silver nanoparticles were produced, with their properties characterised using FTIR and UV-Visible spectroscopies. Turbidimetry determined the Minimum Inhibitory Concentration (MIC) of SNP-MSE, H2O2, and their varied combinations at different concentrations. Agar well diffusion assessed the zone of inhibition. The study comprised three groups: Group 1 assessed SNP-MSE, Group 2 H2O2, and Group 3 their combination. A sample size of 9 (3 per group) was determined with an 80% pretest G power. At 1 μg/ml SNP-MSE, no inhibition zone was observed; for 0.625 mM H2O2, it measured 7.81 ± 0.464 mm; and for the combined treatment, 14.01 ± 0.478 mm. The combined treatment significantly (p=<0.001, p<0.05) outperformed SNP-MSE and H2O2 alone in antibacterial efficacy. Additionally, the Fractional Inhibitory Concentration (FIC) index, below 1, signified synergy between SNP-MSE and H2O2. These findings suggest their collaborative action in eradicating K. pneumoniae. Their synergistic potency proposes a potential novel antibacterial agent, potentially reducing H2O2 side effects, expediting infection recovery, and enhancing overall health.
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