Synergistic antibacterial effect of nisin, ethylenediaminetetraacetic acid, and sulfite on native microflora of fresh white shrimp during ice storage

Abstract

AbstractThis study aims to investigate the effectiveness of using nisin, ethylenediaminetetraacetic acid (EDTA), and sulfite alone or in combination in reducing Vibrio parahaemolyticus, Salmonella enterica, and Pseudomonas fluorescens in broth and native microflora on raw Pacific white shrimp during ice storage. Nisin (50 ppm), EDTA (20 mM), alone or in combination were used to test on the growth of V. parahaemolyticus, S. enterica, and P. fluorescens in broth. Nisin (50 ppm), EDTA (20 mM), sodium metabisulfite (1.25 and 0.625%), ice; alone or in combination were used on shrimps during 1°C storage for 10 days. Microbial and chemical changes were analyzed during shrimp storage. First, the combination of nisin and EDTA exhibited antibacterial effects against V. parahaemolyticus, S. enterica, and P. fluorescens in broth. Second, in shrimp preservation, the combination of nisin, EDTA, and sulfite at a low dose of 0.625% exhibited higher antimicrobial activity than did a high dose of sulfite (1.25%). Based on aerobic bacteria counts, psychrotrophic bacteria, and TVB‐N, shrimp treatment with combination of nisin, EDTA, and low‐dose sulfite were still acceptable within 10 days of storage. Based on our findings, nisin and EDTA can be used to reduce uses of sulfite for shrimp preservation in the future.