Synergistic antibacterial activity of alpha mangostin and resveratrol loaded polymer-based films against bacteria infected wound

Abstract

In general, a wound infection is caused by Staphylococcus aureus (S. aureus) which can be treated by antibacterial agents. Alpha mangostin (M) and resveratrol (R) have been reported to possess antimicrobial properties. However, the M and R combination has not been studied. Thus, this study aimed to develop polymer-based films for the co-delivery of M and R for the treatment of S. aureus infection. M and R were studied for their synergistic antibacterial activity against S. aureus. Various single (ethyl cellulose (EC) and Eudragit® L 100 (L)) and combined polymers (polyvinyl alcohol and chitosan (PVA:CS)) were used to prepare the films. The M and R loaded films were characterized for their physical, chemical and mechanical properties. The M and R release was investigated using Franz diffusion cell. The antibacterial efficacy of the films was assessed against S. aureus. The in vitro cytotoxicity in normal human fibroblast (NHF) was evaluated as well. The results showed that the M and R combination provided synergistic antibacterial activity. The combined PVA:CS polymer showed good mechanical properties and thus was selected to load M and R. The M and R loaded PVA:CS (at a weight ratio of 1:2)-based films provided satisfactory physical, chemical and mechanical properties. Films wetted, swelled and then M and R released at the physiological pH of skin. The rapid bactericidal effect of the developed films was even faster than that of a commercial dressing (Bactigras®). In addition, the films provided low cytotoxicity on NHF cells. Thus, the developed films may serve as a promising drug delivery carrier for the treatment of bacteria infected wound.