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Abstract
Chitosan is very useful in everyday life in adsorption, cosmetics, pharmaceuticals, flocculants, anticancer and antimicrobial.In this study, chitosan was synthesized from chitin extracted from crayfish. The methods such as deproteinization, demineralization, and deacetylation respectively were used in the synthesis of chitosan from crayfish. Antimicrobial activity was studied and it was found that chitosan and Moringaleaf powder were good in inhibiting the growth of microorganisms; confirmed by the results obtained from the experiments. In evaluating the antimicrobial activity, the serial dilution method was used towards Escherichia coli, Staphylococcus aureus, Salmonella typhi, Proteus bulgaris and Streptococcus pneumonia. The antibacterial activity of chitosan composite with the leaf powder of Moringa oleifera Lam., was determined, using well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration determination method. The composites show the synergistic effect at the higher chitosan to lower Moringa concentration and antagonistic effect at higher Moringa to lower chitosan concentrations in all the test organisms. The consequences of this research suggest that the chitosan, Moringa leaf powder, and their composites can be used to discover an antibacterial agent for developing new pharmaceuticals to control studied human pathogenic bacteria responsible for severe illness. Moringa oleiferais widely used in food and folk medicine; while chitosan is widely useful in food, detergents, textiles, leather, paper, pharmaceuticals, and cosmetics industries. Synergism/antagonism of Moringa-chitosan composites was based on concentrations on the tested organisms.Keywords: Antimicrobial, Biopolymer, Health, Synergism/antagonism
Highlights
ABSTRACT:Chitosan is very useful in everyday life in adsorption, cosmetics, pharmaceuticals, flocculants, anticancer and antimicrobial.In this study, chitosan was synthesized from chitin extracted from crayfish
This study is indispensable as this natural biopolymer composites with Moringa oleifera leaf powder is been used on isolates promising to serve as a good antimicrobial agent to those microbes that are resistant to synthetic drugs with low cost without adverse effect on the human system
The residue was washed with demineralized water and the process was followed by deacetylation by adding 50% NaOH for 8Hrs at 60oC and washed with demineralized water to neutral pH.Commercial chitosan was obtained from Germany and compared with the synthesized chitosan
Summary
ABSTRACT:Chitosan is very useful in everyday life in adsorption, cosmetics, pharmaceuticals, flocculants, anticancer and antimicrobial.In this study, chitosan was synthesized from chitin extracted from crayfish. The methods such as deproteinization, demineralization, and deacetylation respectively were used in the synthesis of chitosan from crayfish. This study is indispensable as this natural biopolymer composites with Moringa oleifera leaf powder is been used on isolates promising to serve as a good antimicrobial agent to those microbes that are resistant to synthetic drugs with low cost without adverse effect on the human system. This study aimed at the synergetic/antagonistic antimicrobial potential of chitosan composites with Moringaoliefera leaf powder
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