Abstract

A standardized comparative study measured glucose release and synergistic effects in the solubilization of microcrystalline cellulose by binary mixtures of 11 fungal and bacterial cellulases (eight endoglucanases and three exoglucanases). Evaluation of 16 endo/exo pairs revealed that bacterial/fungal hybrid pairs are very effective in solubilizing microcrystalline cellulose. Of nine bacterial/fungal hybrid pairs studied, six were ranked among the nine most synergistic combinations, and six bacterial/fungal pairs were also among the top nine pairs in terms of soluble-sugar release. One hybrid pair (Acidothermus cellulolyticus E1 and Trichoderma reesei CBH I) was ranked first in both synergism and sugar-release. In exo/exo synergism experiments, the performance of Thermomonospora fusca E3 confirmed its mode of action as "CBH II-like" (i.e., E3 is synergistic with T. reesei CBH I but not with T. reesei CBH II). Studies of endo/endo interactions suggested a possible means of categorizing endoglucanases in terms of substrate specificity.

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