Synergism Activity of Pyoluteorin with Some Antibiotic Against Urinary Tract Infections Pathogens

Abstract

On some pathogenic bacteria isolated from the urinary tract, the antibacterial activity of pyroluteorin in combination with other antibiotics was studied. Pyoluteorin was extracted from Pseudomonas aeruginosa isolated from rhizospheric soil in Baghdad City. Fifty isolates belongs to Urinary Tract Infection were isolated, and the diagnosis was made using cultivars and biochemical tests, and confirmed using Viteck 2 system. Ten isolates each of Escherichia coli and Klebsiella pneumoniae, six of Proteus mirabilis, four of Acinetobacter baumannii, three of Serratia marcescens, and four of Enterobacter cloacae were among the bacterial isolates, gram-positive bacteria including Streptococcus agalactiae (3 isolates), Staphylococcus aureus (6 isolates) and Staphylococcus epidermidis (2 isolates). All isolates were tested for susceptibility test against 10 different antibiotics (Nalidixic acid, Tetracycline, Amoxicillin, Trimethoprin, Ampicillin, salbactam, Norfloxacin, Levofloxacin, ciprofloxacin and Amikacin). The outcomes indicated that 91% and 82% of resistance were reported for nalidixic acid and tetracycline, respectively, whereas 9% of resistance was found for amikacin.