Synergetic effect of Fe/Ag ions incorporated HAp on AZ31 Mg alloy to improve corrosion resistance and osteogenic activity

Abstract

In clinical applications, the rapid deterioration of biodegradable magnesium alloys under in-vitro and in-vivo conditions is a key problem. However, to overcome the limitations surface modification treatments on this material are performed. In this work, a Fe/Ag-HAp coating was developed for biodegradable AZ31 Mg alloy to enhance the corrosion resistance and osteoconductive of the material. The surface morphological studies displayed a flaky-like aggregated structure. In addition to validating the functional groups with Attenuated Total Reflectance Infrared (ATR-IR) spectroscopy and X-Ray Diffraction (XRD) were used to analyze the crystal structure of the samples. Moreover, real-time corrosion was monitored by hydrogen evolution, indicating 2.8 ml of gas was evolved in the coated sample compared with bare material. The potentiodynamic polarization and electrochemical impedance studies revealed the significant corrosion rate and strong impedance resistance of the coating materials. After 7 days of immersion in SBF solution, the coating recruited calcium/phosphate from the entire sample, as observed in the cauliflower-like structure, and the functional group was confirmed by ATR-IR spectroscopy. MTT was performed using MG-63 osteoblast-like cells, the samples were incubated for 24 h and revealed better adhesion on the surface which was observed by SEM. Osteogenic differentiation was evaluated in 3 and 5 days and it showed a significant increase in cell proliferation rate.