Synergetic combination of photoluminescence spectroscopy with microsecond X-ray footprinting

Abstract

X-ray Footprinting uses broadband X-rays for in situ hydroxyl radical labeling to map protein interactions and conformation. High flux density beams are essential to overcome radical scavengers and enhance labeling efficiency. However, conventional sample delivery environments, such as capillary-flow, burns and breaks under the high power density of an unattenuated focused X-ray broadband beam. An alternative is to use a liquid jet, and we have previously demonstrated that use of this container-less form of sample delivery can increase labeling by tenfold at an unfocused X-ray source.