Synchrotron Tomographic Images From Mixed Ground Glass Opacity of Human Lung Adenocarcinoma and Histologic Correlations

Abstract

SESSION TITLE: Lung Cancer II SESSION TYPE: Original Investigation Poster PRESENTED ON: Wednesday, October 26, 2016 at 01:30 PM - 02:30 PM PURPOSE: High resolution tomographic images using synchrotron X-rays are expected to provide reflection for microstructure in detail, thereby to examine the histologic structure without destructing the specimen. This study aims to evaluate the synchrotron tomographic images of mixed ground glass opacity excised on 5mm section in comparison of pathologic examination. METHODS: Phase contrast X-ray images of human lung adenocarcinoma were obtained from the multiple Wiggler beam line 6C at the Pohang Light Source (PLS) in Korea. The X-ray emanated from a bending magnet of the electron storage ring with an electron energy of 2.5 GeV, and a typical beam current was 320mA. Visual images were magnified using 2X and 5X microscope lens, and the distance between the sample and the detector were 85cm and 50cm respectively. Reconstructed tomographic images were compared with images from histologic slides which made from the identical samples. RESULTS: Pulmonary microstructure including terminal bronchiole, alveolar sac, and vasculature could be identified with phase contrast X-ray images. Images from normal lung tissue and mixed ground glass opacity were clearly distinguishable. The hyperplasia of interalveolar septum and dyplasia of microstructure were clearly identified. The imaging findings showed good correlation with those from the histologic examination which obtained from the light microscopic analysis of specimens obtained with hematoxylin-eosin. CONCLUSIONS: Tomographic images which were obtained using synchrotron radiation have the potential for clinical applications. With refinement, this technique may have been used for diagnosis of lung cancer in vivo. CLINICAL IMPLICATIONS: Synchrotron radiation has been used for various medical research including imaing and functional studies. Clinical trials for imaging studies of mammography has been performed and reported excellent results. Recently, synchrotron radiation has been adopted for the radiation therapy, and clinical trials for human brain radiation therapy has been conducted. In lung cancer research, synchrotron radiation can be used for detection of early stage lung cancers, especially for ground glass opacities, which comprises heterogeouns pathologic subtypes. DISCLOSURE: The following authors have nothing to disclose: Eunjue Yi, Sungho Lee, Jae Ho Chung, Sanghoon Jheon No Product/Research Disclosure Information