Abstract
Damage to lower limb muscles requires accurate analysis of the muscular condition via objective microscopic diagnosis. However, microscopic tissue analysis may cause deformation of the tissue structure due to injury induced by external factors during tissue sectioning. To substantiate these muscle injuries, we used synchrotron X-ray imaging technology to project extremely small objects, provide three-dimensional microstructural analysis as extracted samples. In this study, we used mice as experimental animals to create soleus muscle models with various nerve injuries. We morphologically analyzed and quantified the damaged Section and Crush muscles, respectively, via three-dimensional visualization using synchrotron radiation X-ray imaging to diagnose muscle injury. Results of this study can also be used as basic data in the medical imaging field.
Highlights
Damage to lower limb muscles requires accurate analysis of the muscular condition via objective microscopic diagnosis
Among the muscles dominated by the sciatic nerve, the soleus muscles exhibit significant regenerative capacity to promote functional recovery[8,9,10,11]
After injury to the sciatic nerve, muscle weights were measured to confirm the atrophy of the soleus muscle
Summary
Damage to lower limb muscles requires accurate analysis of the muscular condition via objective microscopic diagnosis. Microscopic tissue analysis may cause deformation of the tissue structure due to injury induced by external factors during tissue sectioning To substantiate these muscle injuries, we used synchrotron X-ray imaging technology to project extremely small objects, provide three-dimensional microstructural analysis as extracted samples. We morphologically analyzed and quantified the damaged Section and Crush muscles, respectively, via three-dimensional visualization using synchrotron radiation X-ray imaging to diagnose muscle injury. Existing studies on imaging of this subject have been performed by identifying changes in the muscle shape due to hypertrophy in mice[13], determining the effects of treatment on muscle atrophy[14], and measuring the effects on sciatic neuralgia[15] using microscope or micro-CT. Studies have been conducted using human breast tissue, nails, and hair, and rodent tissues, bones, blood vessels, and muscles using this technology[7,25,26,27,28,29,30,31,32,33]
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