Abstract
New advances in instrumentation, demonstration of proof-of-principle studies, and development of new tools and methods for data analysis and interpretation have enabled the technique of Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy to become a useful tool for structural and functional biology. This paper discusses the characterisation of two new SRCD beamlines, CD1 at the Institute for Storage Rings (ISA), Denmark and 4B8 at the Beijing Synchrotron Radiation Facility (BSRF), China, and new applications of the method for examining biological systems.
Highlights
Circular dichroism (CD) spectroscopy has been a valuable tool in chemical, biochemical and structural biology studies for more than 40 years
This paper describes some of those new applications of Synchrotron Radiation Circular Dichroism (SRCD) and their impact in structural biology
The ratios of the camphor sulphonic acid (CSA) peaks at ∼192.5 and 290 nm measured on CD1 and 4B8 were 2.00 and 1.98, respectively. These values fall within the range of ratios (1.96 to 2.15) measured previously on other SRCD beamlines [5], and correspond closely to the most often-quoted ratio of 2.00 expected for conventional CD (cCD) instruments
Summary
Circular dichroism (CD) spectroscopy has been a valuable tool in chemical, biochemical and structural biology studies for more than 40 years. It is regularly used for examining protein secondary structures, dynamics and folding, monitoring conformational changes associated with ligand binding and macromolecular interactions, and is an essential element of the well-founded biophysics laboratory. SRCD has become a distinct technique in its own right, due to the additional types of studies it enables and the more extensive data that it can produce, relative to conventional CD (cCD) spectroscopy using lab-based commercial instruments [11].
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have