Abstract
The low affinity of composite materials for the hard tissue of human teeth poses a challenge to restorative dentists. This study was undertaken to explore molecular and chemical characteristics of the interface between the dental cement, the buffer layer formed from a next generation biomimetic material that mimics the organic mineral composition of human enamel and dentin, and the intact native hard dental tissue. Seven plane-parallel dental slices were analyzed using synchrotron IR microspectroscopy. The obtained absorption spectra of functional molecular groups were organized into cluster maps. This allowed us to identify the intact tissue, the adhesive agent and the biomimetic layer at their interface and to localize and measure concentrations of functional groups involved in the integration of the biomimetic composite into the hard tissue of the human tooth. The proposed biomimetic material is based on nanocrystal carbonate-substituted calcium hydroxyapatite synthesized from a biogenic calcium source and a complex of basic polar amino acids copying the composition of the human tooth and can form a functional bond with hard dental tissue.
Highlights
The low affinity of composite materials for the hard tissue of human teeth poses a challenge to restorative dentists
By applying IR spectroscopy to the interface between the lightcuring adhesive agent, the biomimetic composite material and natural dental enamel/dentin (Fig. 1B), we were able to identify the major vibrational modes active in the IR spectrum (Fig. 1C) that can be used as spectral fingerprints for the compounds present in the studied sample area
In contrast to the research studies cited in this article, one-dimensional images (IR maps) based on the color codes of absorbance intensity of 4 main spectral ranges (1752– 1704 cm–1, 1718–1358 cm–1, 1269–1224 cm–1, and 1163– 981 cm–1) became a good visual representation of the spatial distribution of molecular groups across the studied sample and of molecular-chemical interactions occurring at the interface between the light-curing material, the biomimetic layer and the enamel/dentin of the human tooth
Summary
The low affinity of composite materials for the hard tissue of human teeth poses a challenge to restorative dentists. This study was undertaken to explore molecular and chemical characteristics of the interface between the dental cement, the buffer layer formed from a generation biomimetic material that mimics the organic mineral composition of human enamel and dentin, and the intact native hard dental tissue. The obtained absorption spectra of functional molecular groups were organized into cluster maps This allowed us to identify the intact tissue, the adhesive agent and the biomimetic layer at their interface and to localize and measure concentrations of functional groups involved in the integration of the biomimetic composite into the hard tissue of the human tooth. С применением метода молекулярной многомерной ИК-визуализации на 7 плоскопараллельных сегментах образцов был выполнен анализ участка интерфейса здоровая твердая ткань (эмаль/дентин) — биомиметический переходной слой — стоматологический материал/адгезивный, созданный с использованием нового поколения биомиметических материалов, воспроизводящих минералорганический комплекс эмали и дентина зубов человека, с нативными твердыми тканями зуба человека и стоматологическим цементом. Ключевые слова: биомиметические материалы, нативная твердая ткань зуба человека, ИК-микроспектроскопия, синхротронное излучение Финансирование: исследование выполнено при поддержке гранта Российского Научного Фонда No 16-15-00003
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