Synchronous recording of load–deformation behaviour and polarized light-microscopic images of the rabbit incisor periodontal ligament during tensile loading

Abstract

Tooth–periodontal ligament–bone segments were cut in the form of rectangular prisms (1.5 mm wide, 0.65 mm thick, and long enough to allow anchorage of the bone and tooth-end portions in a stretching jig) from the mandibular incisors of 10 rabbits. The experimental set-up enabled simultaneous recording, on video, of the changing image brightness under polarizing optics together with extension across the periodontal ligament. Specimens were stretched until failure at a velocity of 0.5 mm/min. The tensile load–deformation curve of the ligament exhibited an initial, non-linear region that was followed by a linear region, a subsequent yielding region preceding the maximum point, and a final descending region. Gradual increases in the intensity of birefringence in the linear and yielding regions indicated that stress concentrations occur in the supporting fibres attached to mineralized tissues. In the final descending region of the curve, progressive breakages of individual fibre bundles occurred, mainly in the middle zone of the ligament. Analysis of the polarized light-microscopic images showed that the increases in brightness and area of birefringent collagen fibre bundles occurred in parallel with the stress generated. These results suggest that the collagen fibre bundles became aligned with the direction of loading and the intensity of their birefringence increased according to the applied tensile force.