Abstract
In cases where the fluorescence of a compound is perturbed by Rayleigh scatter and by stray reflections at the exciting wavelength, the method of synchronous excitation, where the wavelengths of excitation (λ e) and of analysis (λ a) are scanned simultaneously with a constant step, c = λ a - λ e, between them, gives a distinct improvement in the measurement. When the measurement is hampered by this type of stray light and by Raman scatter simultaneously, it is shown that the technique of synchronous excitation again allows an improvement of the measurement compared to conventional techniques, if appropriate values of c are selected. The use of this technique is illustrated for the measurement of the concentration of phenol in aqueous solution; a lower limit of determination of the order of 5 ppb can be obtained.
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