Abstract
1. 1. A 24-hour repeating temperature cycle, consisting of 15 hours at 15 °C, 8 hours at 25 °C, and two 30-minute transition periods, has been used for the synchronization of cell division of Astasia longa grown in 2 per cent proteose peptone and in a chemically defined medium. This results in a burst of cell division every 24 hours for as many as seven generations. 2. 2. Cysteine and methionine were found to be important in the mechanism of synchronization of cells grown in Cramer-Myers medium. Their addition appears to reduce the fission time of these cells without affecting the generation time. 3. 3. The synchronization process is evaluated in terms of a cell cycle. The possible manner in which synchronization is achieved by means of a repeating temperature cycle is discussed.
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