Synaptic vesicle diameters and synaptic cleft widths at the mouse diaphragm in Neonates and Adults

Abstract

Miniature endplate potential (MEPP) amplitude distributions from adult mouse diaphragm junctions show two classes of MEPPs. The larger MEPPs form a bell-shaped distribution (bell-MEPPs) with a variance of 20–30%. The smaller MEPPs form a right-hand skew-distribution composing 1–10% of the MEPPs with a mode 1/10th (s-MEPP class) that of the bell-MEPPs. Junctions of 1-day-old neonates generate mainly s-MEPPs. The MEPP distribution gradually changes to mainly bell-MEPPs during the first 3 weeks of postnatal development. The purpose of this study was to compare the synaptic vesicle diameters and synaptic clefts of 1- to 3-day-old neonatal junctions to those of adults and reinnervated adults. We found no differences between neonates and adults in either synaptic vesicle diameter (56 nm o.d.) or in synaptic cleft widths. Synaptic vesicle diameters were the same for ·touching vesicles’. Junctions of 1-day-old neonates showed no or shallow postsynaptic folds. Postsynaptic folds were essentially developed by the third day although one-quarter of the MEPPs were of the skew-class. Assuming that vesicles are simple containers, we would expect a class of 30-nm o.d. vesicles which would generate the s-MEPP class. Since we found a smooth distribution of synaptic vesicles and no vesicles of 30 nm diameter we conclude that within the constraints of the vesicle hypothesis of transmitter release that s- and bell-MEPPs are from vesicles sized the same and that vesicle volume does not determine the amount of acetylcholine released.