Synaptic and Subsynaptic Localization of NTPDASES 1,2,3,and ECTO- 5'-NUCLEOTIDASE in the Rat Hippocampus and Striatum

Abstract

Event Abstract Back to Event Synaptic and Subsynaptic Localization of NTPDASES 1,2,3,and ECTO- 5'-NUCLEOTIDASE in the Rat Hippocampus and Striatum E. Augusto1, G. P. Cognato1, 2, 3, G. C. Ghisleni1, 3, R. J. Rodrigues1, L. O. Porciuncula2, D. O. Souza2, P. M. Agostinho1*, C. D. Bonan3, J. Sevigny4 and Rodrigo A. Cunha1 1 Faculty of Medicine, University of Coimbra, Center for Neuroscience of Coimbra, Institute of Biochemistry, Portugal 2 Universidade Federal do Rio Grande do Sul, Departamento de Bioquimica, Brazil 3 Faculdade de Biociencias, PUC/RS, Laboratorio de Neuroquimica e Psicofarmacologia, Departamento de Biologia Celular e Molecular, Brazil 4 Centre Hospitalier Universitaire de Quebec, Universite Laval, Centre de Recherche en Rhumatologie et Immunologie, Canada Purinergic signalling in the brain involves ATP and adenosine, both released from neurons and glia and acting through P2 and P1 receptors, respectively. Ecto-nucleotidases play a key role in controlling purinergic signalling since they hydrolyse ATP and can generate adenosine. Although purinergic control of synaptic transmission and plasticity mainly occurs through activation of synaptic receptors, it is not known which ecto- nucleotidases are present at synapses. We now used previously validated antibodies against different ecto- nucleotidases (NTPDase1, 2, 3, and ecto-5’-nucleotidase) to determine their synaptic and sub-synaptic localization in the rat hippocampus and striatum. We found that all ecto-nucleotidases were present in hippocampal and striatal synapses and most were more abundant in synapses than in extra-synaptic fractions. In the hippocampus, NTPDase1 and 5’-nucleotidases were evenly distributed pre- and post- synaptically, whereas NTPDase2 and 3 were more abundant post-synaptically. In the striatum, all ecto- nucleotidases were distributed evenly pre and post-synaptically with the exception of NTPDase3, which was more abundant post-synaptically. However, the set-up of ecto-nucleotidases was different in glutamatergic and GABAergic terminals in both regions; in fact, NTPDase1 was more abundant in GABAergic terminals, whereas NTPDase3 and ecto-5’-nucleotidase were more abundant in glutamatergic terminals and NTPDase2 was evenly distributed in both types of terminals. Overall, these results provide a direct demonstration of the presence and abundance of NTPDase1, 2, 3, and ecto-5’-nucleotidase in central synapses and provide initial evidence that the degradation of ATP and the formation of extracellular adenosine may be differently organized at inhibitory and excitatory synapses.Supported by FCT, CAPES-GRICES and CIHR Conference: 11th Meeting of the Portuguese Society for Neuroscience, Braga, Portugal, 4 Jun - 6 Jun, 2009. Presentation Type: Poster Presentation Citation: Augusto E, Cognato GP, Ghisleni GC, Rodrigues RJ, Porciuncula LO, Souza DO, Agostinho PM, Bonan CD, Sevigny J and Cunha RA (2009). Synaptic and Subsynaptic Localization of NTPDASES 1,2,3,and ECTO- 5'-NUCLEOTIDASE in the Rat Hippocampus and Striatum. Front. Neurosci. Conference Abstract: 11th Meeting of the Portuguese Society for Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.053 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 06 Aug 2009; Published Online: 06 Aug 2009. * Correspondence: P. M Agostinho, Faculty of Medicine, University of Coimbra, Center for Neuroscience of Coimbra, Institute of Biochemistry, Alicante, Portugal, pagost@cnc.cj.uc.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers E. Augusto G. P Cognato G. C Ghisleni R. J Rodrigues L. O Porciuncula D. O Souza P. M Agostinho C. D Bonan J. Sevigny Rodrigo A Cunha Google E. Augusto G. P Cognato G. C Ghisleni R. J Rodrigues L. O Porciuncula D. O Souza P. M Agostinho C. D Bonan J. Sevigny Rodrigo A Cunha Google Scholar E. Augusto G. P Cognato G. C Ghisleni R. J Rodrigues L. O Porciuncula D. O Souza P. M Agostinho C. D Bonan J. Sevigny Rodrigo A Cunha PubMed E. Augusto G. P Cognato G. C Ghisleni R. J Rodrigues L. O Porciuncula D. O Souza P. M Agostinho C. D Bonan J. Sevigny Rodrigo A Cunha Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.