Symptom-oriented network pharmacology revealed the mechanism of HuangQi-DanShen herb pair against cerebral ischemia coupled with comprehensive chemical characterization

Abstract

Ethnopharmacological relevanceIn the clinical practice of traditional Chinese medicine, HuangQi-DanShen (HD) is an important drug pair for the treatment of cerebral ischemia (CI). Aim of the studyElucidate the mechanism of HD against CI based on symptom-oriented network pharmacology coupled with comprehensive chemical characterization. Materials and methodsUHPLC-Q-Exactive Orbitrap-MS technology was firstly used to obtain the chemical profile of HD constituents. A comprehensive strategy combining in-house library, diagnostic ions, Compound Discover software and network databases was then established to identify its chemical constitutes. Symptomatic treatment is a treatment aimed at relieving or eliminating symptoms which is often characterized as a stop-gap measure due to its inability to cure the disease fundamentally. Nevertheless, symptomatic treatment is an indispensable part of clinical practice and has an important place in medical therapeutics. Therefore, network pharmacology technique were used to elucidate molecular mechanisms from the symptoms of CI. Finally, some literatures were further mined to support our conclusions. ResultsA total of 190 ingredients were identified in HD. Symptom-oriented network pharmacology analysis indicated that compounds of HD relieved “blood” through the regulation of ADORA2A, ADORA1, PTPN11, MMP9 and EGFR, relieved “qi” via the regulation of ADORA2A, EGFR, MMP9 and CA2. The therapeutic effect of HD on “faint” was linked to PTPN11 and MMP9, while the regulation of “dyskinesia” was related to ADORA2A and EGFR, and ADORA1, PTPN11 and MMP9 were associated withe its effect on “speech disorder”. ADORA1, ADORA2A and MMP9 were key to the HD component in treating “visual disturbance”. ConclusionThe approach of symptom-oriented network pharmacology coupled with comprehensive chemical characterization proposed a further orientation for exploring the mechanisms of HD against CI.