Sympathetic sprouting into neonatally denervated contralateral target: superior cervical ganglion neuronal numbers and sizes

Abstract

Unilateral superior cervical ganglionectomy results in establishment of an atypical functional projection from the contralateral ganglion to the denervated superior tarsal muscle in neonatal but not in juvenile or adult rats. Previous studies of peripheral nervous system development have attributed enhanced neuroplasticity to either formation of pathways by surplus cells rescued from cell death (hypothanasia-induced hyperplasia), or more vigorous terminal arborization of neurons innervating adjacent target zones (collateral sprouting with associated somatic hypertrophy). In the present study, experiments were performed to determine if neonatal sympathetic sprouting to contralaterally denervated target is associated with superior cervical ganglion neuronal hyperplasia or hypertrophy. Fluoro-Gold injections of right superior tarsal muscle in adult rats following neonatal ganglionectomies labelled cells in the contralateral superior cervical ganglion, whereas cells were labelled strictly ipsilaterally in sham operates. Profile areas of contralaterally projecting neuronal somata were twice as large as those providing ipsilateral innervation. Ganglion volumes, neuronal nuclear diameters and packing densities, and total neuronal numbers in left superior cervical ganglia of animals receiving neonatal right superior cervical ganglionectomy were comparable to those of both right and left ganglia of sham-operated controls. It is concluded that establishment of a contralateral projection does not rescue significant numbers of neurons from naturally occurring cell death. Rather, contralateral sprouting derives from neurons showing pronounced somatic hypertrophy.