Sympathetic neurotransmission in the Watanabe heritable hyperlipidaemic rabbit mesentery artery

Abstract

The aim was to examine sympathetic neurotransmission in the mesenteric artery of atherosclerotic (Watanabe heritable hyperlipidaemic, WHHL) rabbits. Contractile responses to perivascular nerve stimulation (8-64 Hz) and exogenous application of the cotransmitters noradrenaline (0.1-100 microM) and adenosine triphosphate (1 microM) were measured. Catecholamine containing nerves were localised using fluorescence histochemistry. Female WHHL rabbits at 4, 6, and 12 months of age were used, with New Zealand white (NZW) rabbits as controls. The contractile responses to nerve stimulation were significantly smaller in 12 month old WHHL compared to NZW rabbits, although there was no difference at 4 and 6 months of age. Contractile responses of WHHL vessels to noradrenaline (0.1-100 microM) were greater than NZW controls at 4 months, but no differences were seen at 6 and 12 months. Contractile responses to alpha, beta-methylene ATP (0.1 microM) were greater in WHHL rabbits at 6 months but were unchanged at 4 and 12 months. There was no difference in the contractile responses to potassium chloride (120 mM) between 4, 6, and 12 month old WHHL and NZW rabbits. There was no variation in either strain of rabbit in the density of catecholamine containing nerves between 4 and 12 months of age. The reduction of sympathetic neurotransmission in WHHL rabbits at 12 months of age may largely be due to a reduction in the release of the cotransmitters noradrenaline and ATP. The reduced contractile response to nerve stimulation in 12 month old WHHL rabbits is discussed in terms of "protection" of the mesenteric artery from potential vasospasm and atherogenesis.