Sympathetic innervation improves the contractile performance of neonatal cardiac ventricular myocytes in culture

Abstract

We propose that sympathetic innervation could contribute to the improvement in cardiac contractility that normally occurs during neonatal life because these processes are developmentally coincident. Effects of sympathetic innervation were studied in primary cultures of isolated, not previously innervated ventricular cardiomyocytes from neonatal rats. Innervation was produced by addition of autologous neurons from the thoracolumbar sympathetic ganglia, and amplitude and frequency of myocyte contraction were measured by on-line video motion analysis. Sympathetic innervation significantly ( P < 0.0001) increased amplitude of contraction (by 34 ± 8%) and decreased contraction frequency (by 36 ± 3%). The effect of innervation on myocyte contractility was not attenuated by adrenoceptor blockade (10 −6 m propranolol and 10 −6 m phentolamine), but could be reproduced using medium conditioned by cocultures of neurons and myocytes. Sympathetic innervation improves the contractility of isolated cardiomyocytes, indicating that autonomic innervation contributes to maturation of cardiac function.