Sympathetic and Parasympathetic Regulation of Rectal Motility in Rats

Abstract

IntroductionThe colon and rectum are regulated by the autonomic nervous system (ANS). Abnormalities of the ANS are associated with diseases of the colon and rectum while its modulation is a putative mechanism for sacral nerve stimulation. The purpose of this study is to establish a rat model elucidating the role of the efferent ANS on rectal motility. Materials and MethodsRectal motility following transection or stimulation of parasympathetic pelvic nerves (PN) or sympathetic hypogastric nerves (HGN) was measured with rectal strain gauge transducers and quantified as a motility index (MI). Colonic transit was measured 24 hours after transection by calculating the geometric center (GC) of distribution of 51Cr Results and DiscussionTransection of PN and HGN decreased MI to 518 ± 185 g•s (p < 0.05) and increased MI to 5,029 ± 1,954 g•s (p < 0.05), respectively, compared to sham (975 ± 243 g•s). Sectioning of PN and HGN decreased transit with GC = 4.9±0.2 (p < 0.05) and increased transit with GC = 8.1±0.7 (p < 0.02), respectively, compared to sham (GC = 5.8 ± 0.3). Stimulation of PN and HGN increased MI to 831 ± 157% (p < 0.01) and decreased MI to 251 ± 24% (p < 0.05), respectively. ConclusionRectal motility is significantly altered by sectioning or stimulating either HGN or PN. This model may be useful in studying how sacral nerve stimulation exerts its effects and provide insight into the maladies of colonic motility.