Abstract

The symmetric properties of objects extend essentially the possibilities of high resolution research of biological structures in solution by means of X-ray small-angle scattering method. Using this method a number of structural parameters of bacteriophage T7 is determined: icosahedral form of its head, precise dimensions, molecular mass and hydration. Modelling of the intraphage DNA packing is performed in the spherically-symmetric approximation. Changes in the structural organization of the phage caused by environmental alterations (temperature and ionic strength) are investigated and explained using this approximation. Direct restoration of the phage structure with spatial resolution of 12 Å is done assuming axial symmetry of the phage structure.

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