Abstract
In the present study, 20 selected Lactobacillus strains already characterized in a previous research for their capability to grow in conditions simulating the intestinal environment, their resistance to antibiotics, their antibacterial activity and their adhesion capability to intestinal human Caco-2 TC7 and HT-29 MTX cell lines, were further investigated to explore more their probiotic properties. Growth behaviour in the presence of prebiotic (fructooligosac-charides (FOS) and lactulose) at a concentration of 2%, cholesterol removal by measuring the residual cholesterol in medium supplemented with cholesterol, ability to deconjugate bile salts using BSH enzyme and antioxidant activity of culture supernatant of Lactobacillus strains by ABTS·+ and DPPH methods were analyzed. All probiotic strains demonstrated important prebiotic assimilation (P > 0.05) even with OD600 > 3 after 30 h of contact, cholesterol removal ability with maximum percentage of 57% after 24 h of contact and they were found to liberate significantly (P ·+ and DPPH respectively. This study confirmed the suitability of these probiotic strains for application in functional food formulations especially where cholesterol reduction and antioxidant activity in food are needed to assess possible in vivo human health benefits.
Highlights
Identifying microorganisms all over the world and characterizing their metabolites are crucial steps in the study of human nutrition [1]
Growth behaviour in the presence of prebiotic (fructooligosaccharides (FOS) and lactulose) at a concentration of 2%, cholesterol removal by measuring the residual cholesterol in medium supplemented with cholesterol, ability to deconjugate bile salts using bile salt hydrolase (BSH) enzyme and antioxidant activity of culture supernatant of Lactobacillus strains by ABTS.+ and DPPH methods were analyzed
This study confirmed the suitability of these probiotic strains for application in functional food formulations especially where cholesterol reduction and antioxidant activity in food are needed to assess possible in vivo human health benefits
Summary
Identifying microorganisms all over the world and characterizing their metabolites are crucial steps in the study of human nutrition [1]. Milk and dairy products have not been only characterized by their high nutritional value, and by their ability to exert positive effects on the consumer’s health as functional foods [3]. In this context, non digestible ingredients, such as prebiotics, have received important attention, because of their ability to stimulate selectively the growth and activity of probiotic bacteria in the colon [4]. Prebiotics are reported to alleviate the virulence of pathogens like Listeria monocytogenes [8]. This family of compounds includes several oligosaccharides, inulin, lactulose, lactosucrose,... [9]
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