Abstract
The response of selected strains ofRhizobium japonicum to fifty-five antibiotics was determined. The most effective antibiotics for these strains include Kanaymcin, Streptomycin, Dihydrostreptomycin, Triburon, and Viomycin. Mutants were recovered which were resistant to these drugs individually and in various combinations of two. Single and double mutants, which grew as well as the sensitive parent strain on nonselective media, were tested for infectivity and effectiveness of nitrogen-fixation in soybeans. Six weeks after germination, plants were harvested and compared with respect to size, number of nodules, color, number of trifoliate leaves, dry weight, and total nitrogen.
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