SYAUP–CN–26 applies its antifungal activity against Botrytis cinerea by disrupting mitochondrial structure and function

Abstract

This study investigated the effect of SYAUP–CN–26 on mitochondrial structure and function of Botrytis cinerea. The mitochondria, with the addition of SYAUP–CN–26 (EC50 [1.823 mg/L], EC90 [19.263 mg/L], and minimum inhibitory concentration [MIC] [79.754 mg/L]), emerged malformed shape, rough surface and unordered structure. As the concentration of SYAUP–CN–26 increases, the decrease in ATP content and the enhancement in the inhibition of mitochondrial respiratory chain complexes function confirmed that mitochondrial function was disrupted. And the respiratory superposing inhibition showed that SYAUP–CN–26 inhibited the tricarboxylic acid cycle (TCA) pathway of B. cinerea cells. Overall, these results indicated that SYAUP–CN–26 could inhibit mitochondrial structure and function to effect the growth of B. cinerea cells, and inhibition of mitochondrial respiratory chain complexes was a key factor for disruption of B. cinerea mitochondrial function and antifungal activity.