Year-end Sale % OFF 
Abstract
Translational riboswitches are cis-acting RNA regulators that modulate the expression of genes during translation initiation. Their mechanism is considered as an RNA-only gene-regulatory system inducing a ligand-dependent shift of the population of functional ON- and OFF-states. The interaction of riboswitches with the translation machinery remained unexplored. For the adenine-sensing riboswitch from Vibrio vulnificus we show that ligand binding alone is not sufficient for switching to a translational ON-state but the interaction of the riboswitch with the 30S ribosome is indispensable. Only the synergy of binding of adenine and of 30S ribosome, in particular protein rS1, induces complete opening of the translation initiation region. Our investigation thus unravels the intricate dynamic network involving RNA regulator, ligand inducer and ribosome protein modulator during translation initiation.
Highlights
Translational riboswitches are cis-acting RNA regulators that modulate the expression of genes during translation initiation
Messenger RNA and the 30S ribosome form a complex that is stabilized by interactions between messenger RNA (mRNA), ribosomal RNA and ribosomal proteins
We succeed in applying solution NMR spectroscopy of various reconstituted adenine-sensing riboswitch-30S ribosome complexes to investigate both the influence of the inducer ligand adenine and the ribosomal modulator protein rS1 and unravel a synergistic regulatory switch to a functional ON-state
Summary
Translational riboswitches are cis-acting RNA regulators that modulate the expression of genes during translation initiation. Results and discussion We investigated the riboswitch (ASW14–140) containing 18 nucleotides within the coding region of the mRNA as a standby site for ribosome binding (Fig. 1a). The adenine-induced opening of RNA structure in ASW14–140, only affected nucleotides up to position −9, nucleotide A111 (Supplementary Fig. 1).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
