Abstract
AbstractThe looming influenza pandemic has focused attention^1-4^ on the rapid evolution of H5N1 and other human and avian serotypes. The basic tenets of influenza genetics^5^ define gradual changes as drifts caused by point mutations created by a polymerase that lacks a proof reading function. More abrupt changes have been linked to reassortment, which shuffles the eight sub-genomic segments of the influenza genome in dually infected host. The complex evolution of these viruses has created a challenge in vaccine development. Swine influenza isolates from 2003 and 2004 have been identified^6^ that have acquired a human influenza gene, PB1. My analysis of the eight gene segments found large portions of two genes, PB2 and PA, which were identical matches with 1977 swine isolates^7,8^. Additional regions were exact matches with 1998 and 2002 isolates,^9,10^ demonstrating homologous recombination between earlier genomes. The absolute fidelity discounts the role of point mutations in gene drift. Moreover the human PB1 gene represented a reservoir for acquisition of polymorphisms in human seasonal flu. These observations challenge the basic tenets of influenza genetics and provide a method for predicting the changes in seasonal and pandemic influenza, as well as other rapidly evolving genomes.
Highlights
The looming influenza pandemic has focused attention[1,2,3,4] on the rapid evolution of H5N1 and other human and avian serotypes
The human PB1 gene represented a reservoir for acquisition of polymorphisms in human seasonal flu
These observations challenge the basic tenets of influenza genetics and provide a method for predicting the changes in seasonal and pandemic influenza, as well as other rapidly evolving genomes
Summary
The looming influenza pandemic has focused attention[1,2,3,4] on the rapid evolution of H5N1 and other human and avian serotypes. Swine influenza isolates from 2003 and 2004 have been identified[6] that have acquired a human influenza gene, PB1. Seven gene segments of seven recent isolates were compared to swine sequences at the Los Alamos influenza database.
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