Abstract
Hemoglobin-based oxygen carriers (HBOCs) have been developed as a transfusion alternative and oxygen therapy. The Hb source is usually outdated donated human blood or cow blood obtained from cattle industries because of its abundance. This study examined the feasibility of using swine Hb (SHb) for preparation of cellular-type HBOCs, hemoglobin-vesicles (HbV). Purification of SHb from fresh swine whole blood was conducted with processes including carbonylation (SHbO2 ◊ SHbCO), pasteurization (60 °C, 15 hours) and tangential flow ultrafiltration, with yield of 90%. Actually, differential scanning calorimetric analysis showed a denaturation temperature of SHbCO at 83 °C and assures its stability during pasteurization. Concentrated SHbCO together with pyridoxal 5’-phosphate (PLP) as an allosteric effector was encapsulated in phospholipid vesicles to prepare SHbV. After decarbonylation (SHbCO ◊ SHbO2), the oxygen affinity (P50) of SHb changes mainly by PLP, and the influence of Cl‐ was small, in a manner similar to that of human Hb (HHb). However, after encapsulation, vesicles of SHbV showed much lower oxygen affinity (higher P50) than HHbV did. Autoxidation of SHbV was slightly faster than HHbV. Although some differences are apparent in oxygen affinity and autoxidation rates, results clarified that SHb is useful as a starting material for HbV production.
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