Abstract
Several zebrafish strains such as AB, Tübingen (TU), Wild India Kolkata (WIK) and Tupfel long fin (TL) have been established for genetic study. Each strain has its morphological and behavioral traits. Motor traits, however, have not been explored in zebrafish strains. We here applied a treadmill for fish (swimmill) and measured swimming capability of adult zebrafish by critical swimming speed, which is the maximum water velocity in which fish can keep swimming. First, we confirmed that swimming capability does not vary between female and male. Second, we found that the appropriate water temperature for swimming was between 16 and 30 °C. Third, our fin clip experiments using long-finned zebrafish revealed that they can exhibit high swimming capability when the caudal fin length was set between 3 and 10 mm, implying that long-finned zebrafish are unfavorable for fast swimming. Finally, we compared swimming capability of several zebrafish strains and demonstrated that WIK fish was significantly less capable of swimming despite that they have short caudal fin (~9 mm). The offspring of WIK fish were less capable of swimming, while hybrids of WIK and TU showed high swimming performance comparable to TU. Thus, lower swimming capability of WIK strain is inheritable as a motor trait.
Highlights
Several zebrafish strains such as AB, Tübingen (TU), Wild India Kolkata (WIK) and Tupfel long fin (TL) have been established for genetic study
Another report suggested that swimming capability of female and male zebrafish was comparable if they were reared at 25~28 °C, which is the standard condition of zebrafish maintenance in laboratories[5]
We demonstrated that WIK is less capable of swimming compared to the other wild-type strains and that this reduced motor trait is ameliorated in the generation by outcrossing with TU stain, suggesting a genetic component of swimming capability
Summary
Several zebrafish strains such as AB, Tübingen (TU), Wild India Kolkata (WIK) and Tupfel long fin (TL) have been established for genetic study. The other report showed that basal blood glucose level in WIK fish is lower than that in TU fish[11] These evidence with many others indicates that different strains have different genetic traits. We applied the Swim tunnel respirometer to quantitatively analyze Ucrit of adult zebrafish and confirmed that there is no gender difference in swimming capability and that zebrafish can swim well at 16–30 °C water temperature. We demonstrated that WIK is less capable of swimming compared to the other wild-type strains and that this reduced motor trait is ameliorated in the generation by outcrossing with TU stain, suggesting a genetic component of swimming capability
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