Abstract
This work was aimed at evaluating the cardioprotective effects of Castanea sativa Mill. (CSM) bark extract characterized in its phenolic composition by HPLC-DAD-MS analysis. The study was performed using primary cultures of neonatal rat cardiomyocytes to investigate the antioxidant and cytoprotective effects of CSM bark extract and isolated guinea pig left and right atria, left papillary muscle, and aorta to evaluate its direct effect on cholinergic and adrenergic response. In cultured cardiomyocytes the CSM bark extract reduced intracellular reactive oxygen species formation and improved cell viability following oxidative stress in dose-dependent manner. Moreover, the extract decreased the contraction induced by noradrenaline (1 μM) in guinea pig aortic strips and induced transient negative chronotropic and positive inotropic effects without involvement of cholinergic or adrenergic receptors in the guinea pig atria. Our results indicate that CSM bark extract exhibits antioxidant activity and might induce cardioprotective effect.
Highlights
Many natural compounds have a wide range of biological activities including antioxidant, chemopreventive, antiinflammatory, neuroprotective, and cardioprotective effects.Sweet chestnut (Castanea sativa Mill.) is a known source of phenolic bioactive compounds, in particular of tannins [1]
This work was aimed at evaluating the cardioprotective effects of Castanea sativa Mill. (CSM) bark extract characterized in its phenolic composition by HPLC-DAD-MS analysis
Our results indicate that CSM bark extract exhibits antioxidant activity and might induce cardioprotective effect
Summary
Many natural compounds have a wide range of biological activities including antioxidant, chemopreventive, antiinflammatory, neuroprotective, and cardioprotective effects. Sweet chestnut (Castanea sativa Mill.) is a known source of phenolic bioactive compounds, in particular of tannins [1]. Tannins have been classified into two majors groups: hydrolysable and condensed tannins [2]. Hydrolysable tannins can be subdivided into two subclasses: gallotannins and ellagitannins. Gallotannins have a core of β-penta-O-galloyl-D-glucopyranose to which several other galloyl ester groups are linked despite fashion. Ellagitannins are characterized by the presence of two C–C-coupled galloyl ester groups at the 2,3and/or 4,6-positions of a 4C1-glucopyranose [3]
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