Sweet-and-salty biocatalysis: Fructooligosaccharides production using Cladosporium cladosporioides in seawater

Abstract

Production of fructooligosaccharides (FOS) from sucrose was obtained using a bioprocess entirely performed in seawater. The halophilic fungus Cladosporium cladosporioides MUT 5506 was grown in a seawater-based medium and mycelium displayed an optimal activity in seawater at 50–60°C, being stable up to 60°C. Under optimized conditions in seawater (50°C, sucrose 600g/L, lyophilized mycelium 40g/L), C. cladosporioides gave a maximum FOS yield of 344g/L after 72h with a preponderance of 1F-FOS derivatives (1-kestose 184g/L, 1-nystose 98g/L and 1-fructofuranosylnystose 22g/L), and the noteworthy presence of the non-conventional disaccharide blastose (30g/L after 144h). Lyophilized mycelium exhibited good stability in seawater (76% of the initial activity was retained after 15 cycles of reutilization). This proof-of-concept application reports for the first time the production of FOS in a non-conventional medium such as seawater.