Abstract
A microfluidic lactic acid (LA) monitoring system for in-situ monitoring of LA excretion in sweat was fabricated and tested. Our system consists of a micro flow-cell made of PDMS with open flow-channel for sweat sampling and a LA biosensor using redox reaction. The micro flow-cell was fixed on the skin’s surface to connect eccrine sweat gland and micro-channel. Phosphate buffer saline (PBS) was supplied into the flow-channel as carrier flow for transporting whole sweat contents to the biosensor. Continuous monitoring of LA at the skin’s surface was conducted during cycling exercises and intervals. Intensity of the exercises were regulated by heartrates (HR). As a result, secretion of LA was confirmed when the intensity of exercise was high (HR: 159 bpm) and the signal decreased after the exercise. Similar results were obtained in the arm muscle training measurement. This indicates that our system was suitable for the assessment of exercise intensity.
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