Sustained release of human placental extract from chitosan patch incorporated with GO/Zn(Cu)O nanocomposite for enhanced healing of diabetic wounds

Abstract

Chronic wounds are caused due to microbial infections at wound site. Especially, diabetic wounds are a major challenge worldwide. Infection is the major concern in diabetic wound healing. In severe cases, it leads to amputation and death. Human placental extract (HPE) acts as therapeutic agent which possesses anti-inflammatory and angiogenesis property. In this work, HPE was incorporated along with GO/Zn(Cu)O nanocomposite in the wound dressing chitosan patch. It has antibacterial activity and enhances the cell proliferation. The HPE and nanocomposite incorporated polymeric patches are fabricated using gel casting technique. The patch was tested for its antibacterial activity against E. coli, P. aeruginosa, S. typhi, S. flexneri, S. aureus and E. faecium. The PVA/Cs/GO/Zn(Cu)O/HPE patch has swelling percentage of 70 ± 3.5% on 7th day. The water vapour transmission rate (WVTR) for PVA/Cs/GO/Zn(Cu)O/HPE patch is 2249 ± 2.94 g/m2 day−1. The PVA/Cs/GO/Zn(Cu)O/HPE patch shows a porosity of 77 ± 3.8%. In scratch assay the rate of wound closure for PVA/Cs/GO/Zn(Cu)O/HPE patch is 99.4 ± 4.9%. The sustained release of HPE was observed to be 78% at day 14. So far up to our knowledge, sustained release of HPE was not established for diabetic treatment. The biocompatibility and hemocompatability analysis were performed using NIH 3T3 fibroblast cells. In in vivo study the patch shows healing efficiency of 98%. The patch does not cause any toxic effects.