Abstract
The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the present study, porous hydrogels were synthesized using chitosan incorporating latex of the Calotropis procera plant by using a freeze–thaw cycle to stimulate the formation of granulation tissue and angiogenesis in wound healing applications. Structural analysis through Fourier transform infrared (FTIR) spectroscopy confirmed the interaction between chitosan and Calotropis procera. Latex extract containing hydrogel showed slightly higher absorption than the control during water absorption analysis. Thermogravimetric analysis showed high thermal stability of the 60:40 combination of chitosan (CS) and Calotropis procera as compared to all other treatments and controls. A fabricated scaffold application on a chick chorioallantoic membrane (CAM) showed that all hydrogels containing latex extract resulted in a significant formation of blood vessels and regeneration of cells. Overall, the formation of connective tissues and blood capillaries and healing magnitude decreased in ascending order of concentration of extract.
Highlights
IntroductionAs per the World Health Organization (WHO), about 2.8 million people suffered from wounds, and 146,000 deaths occurred due to road traffic accidents and war in Pakistan during 2019 [1]
CS/Cp-LE-based hydrogels were synthesized by freeze-thawing to CS/Cp‐
The present research work explains the growth of new connective tissues and angiogenesis of chitosan-based hydrogels incorporating latex of Calotropis procera
Summary
As per the World Health Organization (WHO), about 2.8 million people suffered from wounds, and 146,000 deaths occurred due to road traffic accidents and war in Pakistan during 2019 [1] For this reason, wound bandages are utilized to treat tissue damage and skin injuries that occur due to accidents, ailments, and surgery [2]. Wound healing comprises combined biochemical and cellular actions directed to recovering the potency of damaged muscles with the restoration of mechanical and efficient reliability [3]. It involves the redevelopment of operative skin illustrated by cooperative and vigorous actions including the formation of an extracellular matrix (ECM)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
