Abstract
Summary Many kinds of cells can be cultured for long periods of time in suspension cultures. Improvements in culture media and cell adaptation have permitted successful cell culture in media supplemented with only small concentrations of serum or with albumin alone, and in a few instances in chemically defined media containing no macromolecules. Sensing and servo units are being developed for the maintenance of optimal cell environment and for the preservation of cell function. Suspension culture techniques for the growth of gram and kilogram amounts of cultured cells are desirable for future genetic, biochemical, and physiological studies.
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