Abstract
Objective: Though it is possible to culture intact mouse follicles to maturity, culture of follicles from larger mammals has not been as successful. We have found that rat follicles grown in traditional flat tissue culture wells often become flattened and lose anatomic integrity just before or just after the acquisition of an antrum. In this study, we have compared the effect of culture on a flat surface with culture in suspension on the anatomic integrity and growth rate of preantral follicles derived from rats.
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