Abstract
Previously, we reported the potent activity of a novel spiropyrimidinetrione, zoliflodacin, against Neisseria gonorrhoeae isolates collected in 2013 from symptomatic men in Nanjing, China. Here, we investigated trends of susceptibilities to zoliflodacin in 986 isolates collected from men between 2014 and 2018. N. gonorrhoeae isolates were tested for susceptibility to zoliflodacin and seven other antibiotics. Mutations in the gyrA, gyrB, parC, parE, and mtrR genes were determined by PCR and sequencing. The MICs of zoliflodacin ranged from ≤0.002 to 0.25 mg/liter; the overall MIC50 and MIC90 were 0.06 mg/liter and 0.125 mg/liter, respectively, in 2018, increasing 2-fold from 2014. However, the percentage of isolates with lower zoliflodacin MICs declined in each year sequentially, while the percentage with higher MICs increased yearly (P ≤ 0.00001). All isolates were susceptible to spectinomycin but resistant to ciprofloxacin (MIC ≥ 1 mg/liter); 21.2% (209/986) were resistant to azithromycin (≥1 mg/liter), 43.4% (428/986) were penicillinase-producing N. gonorrhoeae (PPNG), 26.9% (265/986) were tetracycline-resistant N. gonorrhoeae (TRNG), and 19.4% (191/986) were multidrug-resistant (MDR) isolates. 202 isolates with the lowest (≤0.002 to 0.015 mg/liter) and highest (0.125 to 0.25 mg/liter) zoliflodacin MICs were quinolone resistant with double or triple mutations in gyrA; 193/202 (95.5%) also had mutations in parC There were no D429N/A and/or K450T mutations in GyrB identified in the 143 isolates with higher zoliflodacin MICs; an S467N mutation in GyrB was identified in one isolate. We report that zoliflodacin continues to have excellent in vitro activity against clinical gonococcal isolates, including those with high-level resistance to ciprofloxacin, azithromycin, and extended-spectrum cephalosporins.
Highlights
Neisseria gonorrhoeae, the causative agent of the sexually transmitted infection gonorrhea, has developed resistance to all previously recommended antimicrobial agents for treatment, including sulfonamides, penicillins, tetracyclines and fluoroquinolones[1]
We report that zoliflodacin continues to have excellent in vitro activity against clinical gonococcal isolates, including those with high-level resistance to ciprofloxacin, azithromycin and extended spectrum cephalosporins
Dual antimicrobial therapy with ceftriaxone 250 mg or cefixime 400 mg plus azithromycin 1g is recommended as first-line treatment of uncomplicated gonorrhea by the World Health Organization (WHO)[2] and ceftriaxone plus azithromycin by the U
Summary
The causative agent of the sexually transmitted infection gonorrhea, has developed resistance to all previously recommended antimicrobial agents for treatment, including sulfonamides, penicillins, tetracyclines and fluoroquinolones[1]. Dual antimicrobial therapy with ceftriaxone 250 mg or cefixime 400 mg plus azithromycin 1g is recommended as first-line treatment of uncomplicated gonorrhea by the World Health Organization (WHO)[2] and ceftriaxone plus azithromycin by the U. S. Centers for Disease Control and Prevention (CDC)[3]. Resistance to extended-spectrum cephalosporin (ESCs) and azithromycin is increasing worldwide. Gonococcal isolates with decreased susceptibility to cefixime and/or ceftriaxone have been reported in China[4], Japan[5], Australia[6], European countries[7]
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